化学療法基盤支援活動及びユーザによる評価結果




アッセイの概要
アッセイID: U017報告日: 2011/03/02所属: 三重大学医学部アッセイ者名: 広川佳史アッセイ名: RhoB過剰発現細胞の運動能に与える影響
概要
前立腺がんDU145細胞にRhoBを過剰発現させると細胞の運動能が亢進する。運動能亢進を抑制する阻害剤を同定し、RhoBとの関連を調べるのが目的。細胞をDilにて蛍光標識し、Boyden chamberの底部に阻害剤を入れる。一晩培養し、chamberの裏側に移動した細胞をプレートリーダーで測定する。GSK-3 inhibitorの3種、GSK-3 inhibitor IX ,1-Azakenpaullone, Indirubin-3'-monoxime-5-sulphonic Acidが細胞移動を抑制した。
評価のクライテリア
プレートリーダーによる蛍光測定値が103以下
発表論文
レポート提出者によるレポート内容の最終確認日: -


評価結果の詳細(評価のクライテリアに基づいて陽性と判断された化合物については背景色を黄色にしています.)
WellKIT 1KIT 2KIT 3
compound結果compound結果compound結果
1 - Anone (DMSO)150  none (DMSO)177  none (DMSO)151 
1 - B5-FU141  Pifithrin-a (cyclic)171  ABT-702217 
1 - CBestatin Hydrochloride136  PRIMA-1133  Akt Inhibitor IV137 
1 - DBleomycin sulfate151  Finasteride186  Akt Inhibitor VIII, Isozyme-Selective, Akti-1/2140 
1 - ECisplatin156  Aminoglutethimide172  Akt Inhibitor XI194 
1 - FMethotrexate167  Formestane156  compound C155 
1 - GMitomycin C132  Mifepristone224  ATM/ATR kinase inhibitor307 
1 - HVinblastine sulfate162  TOFA185  ATM kinase inhibitor167 
2 - APaclitaxel118  Amastatin197  Aurora kinase/cdk inhibitor108 
2 - BFlutamide128  Actinonin213  Aurora kinase inhibitor II163 
2 - CDaunorubicin, HCl165  Oligomycin144  Aurora kinase inhibitor III236 
2 - DDoxorubicin, HCl158  Bafilomycin A1107  AG957269 
2 - ETamoxifen, citrate165  HA 14-1219  LFM-A13285 
2 - FActinomycin D134  BH3I-1225  Terreic acid293 
2 - GCamptothecin159  LFM-A13160  KN-93203 
2 - HAclarubicin141  Terreic acid191  KN-62365 
3 - AEtoposide (VP-16)152  E-64d186  Lavendustin C300 
3 - BCytochalasin D124  ALLN149  Kenpaullone140 
3 - C2',5'-dideoxyadenosine128  CA-074170  purvalanol A129 
3 - DAKT inhibitor161  Pepstatin A136  Olomoucine433 
3 - ENL-71-101133  Z-GLF-CMK135  Alsterpaullone, 2-cyanoethyl239 
3 - FAG957141  RS 102895170  Cdk1/2 inhibitor III201 
3 - GKN93149  SB 328437145  Cdk2/9 inhibitor155 
3 - HZ-VAD-FMK137  SB 22500281  NU6102145 
4 - AKenpaullone92  AMD3100 octahydrochloride186  Cdk4 inhibitor226 
4 - BPurvalanol A113  NSC95397203  NSC625987271 
4 - C3-ATA122  SC-ααδ9148  SB218078173 
4 - DOlomoucine133  Amiloride165  isogranulatimide217 
4 - ETBB137  Lidocaine190  Chk2 inhibitor146 
4 - FSulindac sulfide123  Monensin108  Chk2 inhibitor II150 
4 - GValeryl salicylate137  Ouabain82  Ellagic acid (Dihydrate)243 
4 - HNS-398127  Sanguinarine66  TBB239 
5 - ASodium salicylate 143  Glibenclamide169  DMAT373 
5 - BTheophylline127  Dequalinium116  D4476248 
5 - CAzacytidine127  Diazoxide147  TG003234 
5 - DAphidicolin148  Valinomycin93  Diacylglycerol kinase inhibitor II239 
5 - EAG1478152  Nigericin131  IC60211248 
5 - FGenistein123  Diltiazem236  TX-1918277 
5 - GManumycin A161  Nifedipine178  BPIQ-Ⅱ194 
5 - HFTI-276176  Verapamil170  AG1478224 
6 - ASU1498150  PGP-4008132  AG490295 
6 - BGGTI-286171  Fumitremorgin C144  SU4984 
6 - CDexamethasone150  A23187111  SU5402211 
6 - DGSK-3 inhibitor II164  Ionomycin99  Flt-3 Inhibitor193 
6 - EScriptaid145  Thapsigargin87  cFMS Receptor Tyrosine Kinase Inhibitor267 
6 - FTrichostatin A143  t-Butylhydroquinone (BHQ)138  SU6656238 
6 - GAG825177  N-phenylanthranilic acid182  GSK-3 inhibitor IX103 
6 - HCycloheximide153  DIDS106  1-Azakenpaullone105 
7 - ALovastatin201  SB 218078169  Indirubin-3'-monoxime46 
7 - BRadicicol125  Debromohymenialdisine (DBH)167  AG825134 
7 - C17-AAG114  Rotenone119  AG1024209 
7 - DAG1024194  Antimycin A194  AGL 2263155 
7 - E1400W, HCl172  Leptomycin B*162  BMS-345541428 
7 - FAMT, HCl166  R59022166  IKK-2 inhibitor VI123 
7 - GAG490164  Dioctanoylglycol151  IRAK-1/4 inhibitor314 
7 - HCucurbitacin I155  RHC80267130  JAK Inhibitor I244 
8 - ASP600125140  Xanthohumol110  JAK3 Inhibitor VI120 
8 - BDamnacanthal152  C75169  SP600125223 
8 - CPD 98059172  Cerulenin122  JNK inhibitor VIII269 
8 - DU0126149  Tunicamycin85  Damnacanthal155 
8 - EFumagillin182  Deoxynojirimycin188  PP2119 
8 - FGM 6001168  Swainsonine185  ERK inhibitor II235 
8 - GN-Acetyl-L-cysteine181  LY 83583113  PD98059209 
8 - HAminoguanidine, HCl195  ODQ229  U-0126110 
9 - AL-NMMA165  Anacardic acid126  MEK inhibitor I178 
9 - BPD169316136  Chetomin66  SU11274130 
9 - CSB 203580176  Dimethyloxalylglycine192  ML-7189 
9 - DRapamycin178  HR22C16104  SB20219087 
9 - ENU1025185  Monastrol150  SB239063145 
9 - FBenzamide168  Nordihydroguaiaretic acid (NDGA)130  AG1296132 
9 - GD609164  ETYA192  SU1165290 
9 - HIBMX165  Baicalein147  PDGF receptor tyrosine kinase inhibitor V97 
10 - ARo-20-1724159  Nutlin-3179  PDGF receptor tyrosine kinase inhibitor IV102 
10 - BZaprinast170  MDM2 inhibitor179  LY-294002162 
10 - CAG1296170  Phenelzine166  Wortmannin78 
10 - DLY294002168  Deprenyl182  H-89464 
10 - EWortmannin148  Decylubiquinone189  4-cyano-3-methylisoquinoline243 
10 - FH-89, HCl160  Ro 5-4864264  Bisindolymaleimide I, HCl92 
10 - GBisindolymaleimide I, HCl128  Lonidamine210  Go7874134 
10 - HH-7166  ML-7137  Rp-8-CPT-cGMPS376 
11 - AStaurosporine135  Benzylguanine211  KT5823244 
11 - BcPLA2inhibitor144  DFMO203  PKR inhibitor67 
11 - COBAA141  KT 5823176  RAF1 kinase inhibitor I244 
11 - DCantharidin169  Rp-8-CPT-cGMPS269  ZM 336372239 
11 - ECytostatin185  MK 886184  H-1152269 
11 - FCyclosporin A149  Clofibrate230  Y-27632303 
11 - GFK-506155  BADGE167  radicicol95 
11 - HMG-132157  Troglitazone170  PP1 analog167 
12 - ALactacystin144  AZT337  Syk inhibitor169 
12 - BHydroxyurea150  Nalidixic acid174  SB431542190 
12 - CHA1077137  a-Amanitin66  TGF-b RI kinase inhibitor II267 
12 - DY27632143  MST-312207  Tpl2 kinase inhibitor203 
12 - EPP1 (analog)165  b-Rubromycin111  TrkA inhibitor123 
12 - FPP-H146  SB 431542170  VEGFR receptor tyrosine kinase inhibitor II167 
12 - GNocodazole143  N1,N12-Diethylspermine (BESpm)126  VEGF recptor 2 kinase inhibitor I254 
12 - HDephostatin155  Fumonisin B1268  SU1498193