化学療法基盤支援活動及びユーザによる評価結果




アッセイの概要
アッセイID: U013報告日: 2009/05/25所属: 弘前大学医学研究科小児科学講座アッセイ者名: 伊藤悦朗アッセイ名: WST-8 assay (CMK11-5)
概要
本研究では標準阻害剤キットIおよびIIの試薬を細胞培養系(骨髄性白血病細胞株)に添加し、細胞増殖に対する影響を調べた。 細胞増殖は同仁化学 Cell Counting Kit-8を用いて測定した。K562, HEL, CMK11-5細胞株における阻害剤の細胞増殖抑制作用のデータをエクセル・ファイルに記載し報告する。
評価のクライテリア
発表論文
レポート提出者によるレポート内容の最終確認日: -


評価結果の詳細(評価のクライテリアに基づいて陽性と判断された化合物については背景色を黄色にしています.)
WellKIT 1KIT 2
compound結果compound結果
1 - Anone (DMSO)100.0  none (DMSO)100.0 
1 - B5-FU78.6  Pifithrin-a (cyclic)99.8 
1 - CBestatin Hydrochloride128.4  PRIMA-1103.5 
1 - DBleomycin sulfate78.8  Finasteride108.6 
1 - ECisplatin109.6  Aminoglutethimide98.6 
1 - FMethotrexate54.7  Formestane114.9 
1 - GMitomycin C10.4  Mifepristone101.6 
1 - HVinblastine sulfate5.3  TOFA134.7 
2 - APaclitaxel69.6  Amastatin111.9 
2 - BFlutamide104.6  Actinonin87.4 
2 - CDaunorubicin, HCl24.3  Oligomycin-0.2 
2 - DDoxorubicin, HCl41.2  Bafilomycin A10.2 
2 - ETamoxifen, citrate8.9  HA 14-1100.5 
2 - FActinomycin D33.3  BH3I-197.9 
2 - GCamptothecin6.0  LFM-A13118.6 
2 - HAclarubicin22.7  Terreic acid122.3 
3 - AEtoposide (VP-16)23.4  E-64d118.6 
3 - BCytochalasin D53.5  ALLN7.9 
3 - C2',5'-dideoxyadenosine116.4  CA-074100.0 
3 - DAKT inhibitor121.2  Pepstatin A100.0 
3 - ENL-71-101128.0  Z-GLF-CMK95.1 
3 - FAG957112.3  RS 102895110.2 
3 - GKN93106.5  SB 328437106.0 
3 - HZ-VAD-FMK119.0  SB 22500241.2 
4 - AKenpaullone93.3  AMD3100 octahydrochloride116.7 
4 - BPurvalanol A76.6  NSC95397121.6 
4 - C3-ATA95.2  SC-ααδ996.3 
4 - DOlomoucine92.8  Amiloride94.7 
4 - ETBB94.2  Lidocaine89.1 
4 - FSulindac sulfide92.8  Monensin14.9 
4 - GValeryl salicylate96.1  Ouabain14.9 
4 - HNS-398110.8  Sanguinarine-1.9 
5 - ASodium salicylate 116.6  Glibenclamide112.1 
5 - BTheophylline107.7  Dequalinium29.3 
5 - CAzacytidine6.7  Diazoxide95.1 
5 - DAphidicolin74.0  Valinomycin5.6 
5 - EAG147880.5  Nigericin8.8 
5 - FGenistein108.2  Diltiazem101.2 
5 - GManumycin A130.8  Nifedipine90.5 
5 - HFTI-276108.4  Verapamil88.1 
6 - ASU149890.6  PGP-4008119.8 
6 - BGGTI-286102.7  Fumitremorgin C90.7 
6 - CDexamethasone92.0  A231872.3 
6 - DGSK-3 inhibitor II112.5  Ionomycin5.3 
6 - EScriptaid14.5  Thapsigargin8.4 
6 - FTrichostatin A16.4  t-Butylhydroquinone (BHQ)114.0 
6 - GAG825101.7  N-phenylanthranilic acid107.9 
6 - HCycloheximide51.6  DIDS117.4 
7 - ALovastatin93.7  SB 21807850.9 
7 - BRadicicol49.9  Debromohymenialdisine (DBH)92.3 
7 - C17-AAG53.5  Rotenone35.6 
7 - DAG1024101.9  Antimycin A1102.8 
7 - E1400W, HCl105.1  Leptomycin B*21.2 
7 - FAMT, HCl103.1  R59022103.7 
7 - GAG490126.5  Dioctanoylglycol107.0 
7 - HCucurbitacin I6.3  RHC8026799.5 
8 - ASP60012551.6  Xanthohumol80.9 
8 - BDamnacanthal123.1  C7599.5 
8 - CPD 98059130.8  Cerulenin99.8 
8 - DU012688.2  Tunicamycin27.9 
8 - EFumagillin114.7  Deoxynojirimycin92.6 
8 - FGM 6001102.4  Swainsonine97.9 
8 - GN-Acetyl-L-cysteine114.7  LY 8358317.4 
8 - HAminoguanidine, HCl129.4  ODQ70.2 
9 - AL-NMMA123.9  Anacardic acid110.9 
9 - BPD169316110.6  Chetomin21.6 
9 - CSB 20358098.8  Dimethyloxalylglycine94.0 
9 - DRapamycin74.7  HR22C1667.0 
9 - ENU1025106.3  Monastrol92.8 
9 - FBenzamide114.0  Nordihydroguaiaretic acid (NDGA)108.8 
9 - GD609117.8  ETYA123.0 
9 - HIBMX106.0  Baicalein86.0 
10 - ARo-20-1724133.3  Nutlin-3101.6 
10 - BZaprinast95.7  MDM2 inhibitor97.7 
10 - CAG129699.3  Phenelzine93.7 
10 - DLY29400264.8  Deprenyl105.8 
10 - EWortmannin89.6  Decylubiquinone106.7 
10 - FH-89, HCl89.2  Ro 5-486494.4 
10 - GBisindolymaleimide I, HCl53.0  Lonidamine101.6 
10 - HH-7141.9  ML-784.4 
11 - AStaurosporine7.7  Benzylguanine96.0 
11 - BcPLA2inhibitor107.5  DFMO113.0 
11 - COBAA103.6  KT 582367.9 
11 - DCantharidin9.9  Rp-8-CPT-cGMPS109.3 
11 - ECytostatin22.9  MK 886106.0 
11 - FCyclosporin A16.9  Clofibrate116.5 
11 - GFK-506133.5  BADGE105.6 
11 - HMG-1323.9  Troglitazone102.3 
12 - ALactacystin145.3  AZT118.8 
12 - BHydroxyurea124.8  Nalidixic acid102.1 
12 - CHA107798.3  a-Amanitin52.6 
12 - DY2763297.1  MST-31279.1 
12 - EPP1 (analog)118.6  b-Rubromycin25.3 
12 - FPP-H126.3  SB 431542135.1 
12 - GNocodazole29.9  N1,N12-Diethylspermine (BESpm)117.0 
12 - HDephostatin107.5  Fumonisin B1121.4