化学療法基盤支援活動及びユーザによる評価結果




アッセイの概要
アッセイID: U012報告日: 2009/05/25所属: 弘前大学医学研究科小児科学講座アッセイ者名: 伊藤悦朗アッセイ名: WST-8 assay (HEL)
概要
本研究では標準阻害剤キットIおよびIIの試薬を細胞培養系(骨髄性白血病細胞株)に添加し、細胞増殖に対する影響を調べた。 細胞増殖は同仁化学 Cell Counting Kit-8を用いて測定した。K562, HEL, CMK11-5細胞株における阻害剤の細胞増殖抑制作用のデータをエクセル・ファイルに記載し報告する。
評価のクライテリア
発表論文
レポート提出者によるレポート内容の最終確認日: -


評価結果の詳細(評価のクライテリアに基づいて陽性と判断された化合物については背景色を黄色にしています.)
WellKIT 1KIT 2
compound結果compound結果
1 - Anone (DMSO)100.0  none (DMSO)100.0 
1 - B5-FU78.3  Pifithrin-a (cyclic)86.6 
1 - CBestatin Hydrochloride92.6  PRIMA-191.8 
1 - DBleomycin sulfate70.2  Finasteride97.0 
1 - ECisplatin91.9  Aminoglutethimide93.1 
1 - FMethotrexate65.9  Formestane104.3 
1 - GMitomycin C24.4  Mifepristone82.8 
1 - HVinblastine sulfate38.0  TOFA105.6 
2 - APaclitaxel59.3  Amastatin94.8 
2 - BFlutamide82.2  Actinonin92.7 
2 - CDaunorubicin, HCl12.8  Oligomycin6.9 
2 - DDoxorubicin, HCl4.7  Bafilomycin A1-1.3 
2 - ETamoxifen, citrate8.9  HA 14-190.9 
2 - FActinomycin D26.0  BH3I-194.0 
2 - GCamptothecin-2.3  LFM-A1396.6 
2 - HAclarubicin10.9  Terreic acid68.5 
3 - AEtoposide (VP-16)31.4  E-64d131.9 
3 - BCytochalasin D46.5  ALLN27.2 
3 - C2',5'-dideoxyadenosine94.2  CA-074100.9 
3 - DAKT inhibitor108.9  Pepstatin A97.0 
3 - ENL-71-10196.1  Z-GLF-CMK67.7 
3 - FAG95797.7  RS 102895106.0 
3 - GKN9381.8  SB 328437111.2 
3 - HZ-VAD-FMK105.4  SB 22500265.9 
4 - AKenpaullone36.0  AMD3100 octahydrochloride113.8 
4 - BPurvalanol A44.6  NSC95397126.7 
4 - C3-ATA85.7  SC-ααδ9115.1 
4 - DOlomoucine84.9  Amiloride91.8 
4 - ETBB78.7  Lidocaine88.4 
4 - FSulindac sulfide82.9  Monensin38.4 
4 - GValeryl salicylate82.6  Ouabain10.3 
4 - HNS-398100.0  Sanguinarine-5.2 
5 - ASodium salicylate 98.4  Glibenclamide106.9 
5 - BTheophylline82.2  Dequalinium75.0 
5 - CAzacytidine3.1  Diazoxide106.9 
5 - DAphidicolin39.5  Valinomycin12.1 
5 - EAG147869.4  Nigericin23.7 
5 - FGenistein83.7  Diltiazem105.6 
5 - GManumycin A88.8  Nifedipine99.1 
5 - HFTI-27689.9  Verapamil89.2 
6 - ASU149813.2  PGP-4008111.6 
6 - BGGTI-28684.9  Fumitremorgin C104.7 
6 - CDexamethasone84.1  A231874.7 
6 - DGSK-3 inhibitor II86.4  Ionomycin11.2 
6 - EScriptaid6.2  Thapsigargin18.1 
6 - FTrichostatin A8.9  t-Butylhydroquinone (BHQ)56.5 
6 - GAG82583.3  N-phenylanthranilic acid95.7 
6 - HCycloheximide24.0  DIDS100.0 
7 - ALovastatin92.2  SB 21807841.8 
7 - BRadicicol50.4  Debromohymenialdisine (DBH)96.1 
7 - C17-AAG48.4  Rotenone68.5 
7 - DAG1024122.1  Antimycin A197.0 
7 - E1400W, HCl83.3  Leptomycin B*35.8 
7 - FAMT, HCl83.7  R59022103.4 
7 - GAG49093.4  Dioctanoylglycol131.0 
7 - HCucurbitacin I5.8  RHC8026796.6 
8 - ASP60012565.5  Xanthohumol96.6 
8 - BDamnacanthal93.8  C75113.4 
8 - CPD 9805996.9  Cerulenin116.4 
8 - DU012689.9  Tunicamycin18.1 
8 - EFumagillin84.9  Deoxynojirimycin94.4 
8 - FGM 600194.2  Swainsonine100.4 
8 - GN-Acetyl-L-cysteine97.7  LY 8358322.0 
8 - HAminoguanidine, HCl90.7  ODQ83.2 
9 - AL-NMMA105.8  Anacardic acid108.6 
9 - BPD16931686.8  Chetomin22.4 
9 - CSB 20358093.4  Dimethyloxalylglycine97.4 
9 - DRapamycin69.4  HR22C1678.9 
9 - ENU102595.7  Monastrol105.2 
9 - FBenzamide88.8  Nordihydroguaiaretic acid (NDGA)111.2 
9 - GD609102.7  ETYA106.0 
9 - HIBMX90.7  Baicalein90.1 
10 - ARo-20-1724108.9  Nutlin-3104.7 
10 - BZaprinast79.8  MDM2 inhibitor76.7 
10 - CAG129669.4  Phenelzine119.4 
10 - DLY29400275.2  Deprenyl109.1 
10 - EWortmannin79.1  Decylubiquinone116.8 
10 - FH-89, HCl77.5  Ro 5-4864109.9 
10 - GBisindolymaleimide I, HCl36.0  Lonidamine111.2 
10 - HH-7104.7  ML-789.7 
11 - AStaurosporine8.1  Benzylguanine126.3 
11 - BcPLA2inhibitor93.4  DFMO115.1 
11 - COBAA80.6  KT 582381.9 
11 - DCantharidin27.1  Rp-8-CPT-cGMPS107.8 
11 - ECytostatin38.8  MK 886103.0 
11 - FCyclosporin A17.8  Clofibrate130.2 
11 - GFK-506100.4  BADGE100.9 
11 - HMG-1323.9  Troglitazone101.3 
12 - ALactacystin114.7  AZT121.1 
12 - BHydroxyurea103.9  Nalidixic acid119.0 
12 - CHA1077108.9  a-Amanitin47.0 
12 - DY27632111.2  MST-31221.1 
12 - EPP1 (analog)105.0  b-Rubromycin65.1 
12 - FPP-H111.2  SB 431542146.6 
12 - GNocodazole63.6  N1,N12-Diethylspermine (BESpm)110.3 
12 - HDephostatin99.2  Fumonisin B175.9